ATP Can Efficiently Stabilize Protein through a Unique Mechanism
Xinwen Ou, Yichong Lao, Jingjie Xu, Yanee Wutthinitikornkit, Rui Shi, Xiangjun Chen, Jingyuan Li
Abstract
at higher concentration (150 mM under physiological conditions) can similarly mediate the formation of the ATP cluster on protein. The ATP cluster can effectively reduce the fluctuations of the vulnerable region and thus stabilize the protein against thermal perturbations. Both ATP binding and the considerable improvement of thermal stability of ATP-bound protein were verified by experiments.
Topics & Concepts
BiophysicsChemistryLysozymeMolecular dynamicsAdenosine triphosphateCluster (spacecraft)ChemiosmosisMalate dehydrogenaseBiochemistryEnzymeATP synthaseBiologyComputational chemistryProgramming languageComputer scienceProtein Structure and DynamicsEnzyme Structure and FunctionSpectroscopy and Quantum Chemical Studies