Doxorubicin-loaded polymeric nanoparticles containing ketoester-based block and cholesterol moiety as specific vehicles to fight estrogen-dependent breast cancer
Paweł Misiak, Katarzyna Niemirowicz-Laskowska, Karolina H. Markiewicz, Przemysław Wielgat, Izabela Kurowska, Robert Czarnomysy, Iwona Misztalewska‐Turkowicz, Halina Car, Krzysztof Bielawski, Agnieszka Z. Wilczewska
Abstract
Abstract The presented research concerns the preparation of polymer nanoparticles (PNPs) for the delivery of doxorubicin. Several block and statistical copolymers, composed of ketoester derivative, N -isopropylacrylamide, and cholesterol, were synthesized. In the nanoprecipitation process, doxorubicin (DOX) molecules were kept in spatial polymeric systems. DOX-loaded PNPs show high efficacy against estrogen-dependent MCF-7 breast cancer cell lines despite low doses of DOX applied and good compatibility with normal cells. Research confirms the effect of PNPs on the degradation of the biological membrane, and the accumulation of reactive oxygen species (ROS), and the ability to cell cycle arrest are strictly linked to cell death. Graphical Abstract