Application of <scp>l</scp>-Threonine Aldolase to on-DNA Reactions
Jing Chai, Xiaojie Lu, Christopher C. Arico-Muendel, Yun Ding, Michael P. Pollastri
Abstract
Enzymatic catalysis is a highly attractive approach to the DNA encoded library technology (DEL) that has not been widely explored. In this paper, we report an l-threonine aldolase (l-TA)-catalyzed on-DNA aldol reaction to form β-hydroxy-α-amino acids, and its diastereoselectivity determination. l-TAs from three species show good on-DNA aldehyde scope and complementary stereoselectivity. The formed aldol product can be further diversified via various reactions, which demonstrates the utility of this reaction in DEL.
Topics & Concepts
ChemistryAldol reactionAldolase AAldehydeDNAThreonineCatalysisEnzymeStereoselectivityCombinatorial chemistryBiochemistryStereochemistrySerineChemical Synthesis and AnalysisAmino Acid Enzymes and MetabolismPolyamine Metabolism and Applications