Detecting KPC-2 and NDM-1 Coexpression in Klebsiella pneumoniae Complex from Human and Animal Hosts in South America
Felipe Vásquez‐Ponce, Karine Dantas, Johana Becerra, Gregory Melocco, Fernanda Esposito, Brenda Cardoso, Larissa Rodrigues, Keila Cruz Lima, Aline Valério de Lima, Fábio P. Sellera, Renata Mattos, Lucas Trevisoli, Marco A. Vianello, Thaís Cristine Marques Sincero, José Di Conza, Eliana Carolina Vespero, Gabriel Gutkind, Jorge Luiz Mello Sampaio, Nilton Lincopán
Abstract
in Latin America and the Caribbean have recently been issued by PAHO and WHO, emphasizing the importance of appropriate microbiological diagnosis and the effective and articulated implementation of infection prevention and control programs. In this study, we evaluated methods based on inhibition of ceftazidime (CAZ), ceftazidime-avibactam (CZA), and aztreonam (ATM) by dipicolinic acid (DPA), EDTA, and avibactam (AVI) inhibitors for the identification of KPC-2- and NDM-1-coexpression in members of the K. pneumoniae complex recovered from human and animal hosts. Our results demonstrate that inhibition by AVI, DPA, and EDTA may provide simple and inexpensive methods for the presumptive detection of coexpression of KPC-2 and NDM-1 in members of the K. pneumoniae complex.