On the dilemma of using single EV analysis for liquid biopsy: the challenge of low abundance of tumor EVs in blood
Meruyert Imanbekova, Mohul Sharma, Sebastian Wachsmann‐Hogiu
Abstract
Single extracellular vesicle (EV) analysis holds great promise for non-invasive cancer diagnostics, offering insights into tumor-specific biomarkers and enabling personalized treatment strategies.However, a significant challenge in the path towards clinical applications is the low abundance of tumor-derived EVs (tEVs) in biofluids, which reduces the sensitivity, specificity, and accuracy of detection.This review emphasizes the importance of analyzing a large number of single EVs to overcome this limitation.We estimate that less than 0.1% of total EVs could be from cancer cells in a mixed sample.Additionally, the development of more efficient tEVs isolation methods and targeted enrichment strategies, as well as high-throughput analysis techniques are crucial for improving diagnostic accuracy and advancing liquid biopsy applications in cancer care.