Commentary on human pluripotent stem cell-based blood–brain barrier models
Ethan S. Lippmann, Samira M. Azarin, Sean P. Palecek, Eric V. Shusta
Abstract
In 2012, we provided the first published evidence that human pluripotent stem cells could be differentiated to cells exhibiting markers and phenotypes characteristic of the blood-brain barrier (BBB). In the ensuing years, the initial protocols have been refined, and the research community has identified both positive and negative attributes of this stem cell-based BBB model system. Here, we give our perspective on the current status of these models and their use in the BBB community, as well as highlight key attributes that would benefit from improvement moving forward.
Topics & Concepts
Induced pluripotent stem cellHuman Induced Pluripotent Stem CellsStem cellNeuroscienceBlood–brain barrierNeurologyMedicinePerspective (graphical)BiologyBioinformaticsComputational biologyCell biologyEmbryonic stem cellComputer scienceGeneCentral nervous systemArtificial intelligenceGeneticsPluripotent Stem Cells ResearchBarrier Structure and Function StudiesSingle-cell and spatial transcriptomics