The first report of the blaIMP-10 gene and complete sequence of the IMP-10-encoding plasmid p12NE515 from Pseudomonas aeruginosa in China
Wei Feng, Jun Lv, Hongping Wang, Pu Yao, Lirong Xiong, Peiyuan Xia, Qian Yuan, Fengjun Sun
Abstract
Objective To investigate a detailed genomic characterization of the bla IMP-10 -carrying plasmid p12NE515 from a Pseudomonas aeruginosa isolate in China. Methods Plasmid p12NE515 was subjected to whole-genome sequencing and the complete sequence was compared with related plasmid sequences. Transferability of plasmid, carbapenemase activity and bacterial susceptibility profiles were determined to assess p12NE515-mediated resistance phenotypes. Results P. aeruginosa 12NE515 was identified as a less common sequence type of ST1976. p12NE515 harboring bla IMP-10 possessed a backbone identical to plasmid p60512-IMP (carrying bla IMP-1 ), but the accessory resistance regions differed. Only one accessory module, Tn 7339 , was carried in p12NE515, and this transposon was an insertion sequence-mediated transposition unit generated by the insertion of a novel class 1 integron, In1814, at the downstream end of IS Pa17 . Here, bla IMP-10 together with aacA7 was located in In1814, being at evolution stage III of Tn 402 -associated integron due to truncation of the tni module. Conclusion This study is the first to determine the complete sequence of a bla IMP-10 -carrying plasmid, and this is also the first report of a bla IMP-10 -producing strain in China. The prevalence of the bla IMP-10 gene and the genetic characterization of the bla IMP-10 -carrying plasmid should be analyzed to provide deeper insight into the transmission mechanism of antimicrobial resistance genes.