Urate oxidase loaded in PCN-222(Fe) with peroxidase-like activity for colorimetric detection of uric acid
Xiao Liang, Yingxuan Chen, Kai Wen, Haobo Han, Quanshun Li
Abstract
to generate a blue product with a maximum absorbance at 652 nm. Under optimal conditions, the increasing absorbance at 652 nm was proportional to the UA concentration. The linear range for UA detection was 10-800 μM with a limit of detection of 3.5 μM, and the cascade system has been successfully utilised for the detection of UA in human serum samples to demonstrate the accuracy of the proposed method. In conclusion, the cascade catalytic system based on the immobilization of UOx in PCN-222(Fe) has the potential to be used as a rapid and sensitive sensor for UA detection.
Topics & Concepts
Urate oxidaseUric acidPeroxidaseChemistryBiochemistryOxidase testEnzymeChromatographyAdvanced Nanomaterials in CatalysisAdvanced biosensing and bioanalysis techniquesElectrochemical sensors and biosensors