Optical 4D oxygen mapping of microperfused tissue models with tunable <i>in vivo</i> -like 3D oxygen microenvironments
Milan Finn Wesseler, Mathias Nørbæk Johansen, Aysel Kızıltay, Kim I. Mortensen, Niels B. Larsen
Abstract
embedded commercially available oxygen microsensor beads read out by confocal phosphorescence lifetime microscopy (PLIM). Optimal acquisition conditions and data analysis procedures are established and implemented in a publicly available software package. The versatility of the established method is first demonstrated in model-assisted fluidic design of microperfused 3D printed hydrogel culture chips with the aim of full culture oxygenation, and subsequently for monitoring and maintenance of physiologically relevant spatial and temporal oxygen gradients in the 3D printed chips controlled by static or dynamic flow conditions during 3D culture.
Topics & Concepts
OxygenOxygen tension3D cell cultureConfocal microscopyIn situBiomedical engineeringChemistryLimiting oxygen concentrationMaterials scienceBiophysicsComputer scienceBiological systemIn vitroCell biologyEngineeringBiochemistryBiologyOrganic chemistry3D Printing in Biomedical ResearchAnalytical Chemistry and SensorsInnovative Microfluidic and Catalytic Techniques Innovation