Monitoring tumor cell death in murine tumor models using deuterium magnetic resonance spectroscopy and spectroscopic imaging
Friederike Hesse, Vencel Somai, Felix Kreis, Flaviu Bulat, Alan J. Wright, Kevin M. Brindle
Abstract
Significance There is an unmet clinical need for sensitive methods for detecting cell death in vivo, for example, in disease and following tumor treatment. We show here that deuterium magnetic resonance measurements at 7 T of labeled malate production from injected 2 H-labeled fumarate provide a sensitive method for detecting tumor cell death in vivo following treatment. Malate production was relatively slow in viable cells but was markedly increased in necrotic tissue.
Topics & Concepts
In vivoProgrammed cell deathNuclear magnetic resonanceNuclear magnetic resonance spectroscopyMagnetic resonance imagingCellChemistryTumor cellsCancer researchPathologyBiologyMedicineApoptosisBiochemistryRadiologyPhysicsBiotechnologyAdvanced NMR Techniques and ApplicationsAdvanced MRI Techniques and ApplicationsElectron Spin Resonance Studies