Zwitterionic polymers-armored amyloid-like protein surface combats thrombosis and biofouling
Xiaohui Mou, Miao Wan, Wentai Zhang, Wenxuan Wang, Qing Ma, Zeyu Du, Xin Li, Nan Huang, Zhilu Yang
Abstract
Proteins, cells and bacteria adhering to the surface of medical devices can lead to thrombosis and infection, resulting in significant clinical mortality. Here, we report a zwitterionic polymers-armored amyloid-like protein surface engineering strategy we called as “armored-tank” strategy for dual functionalization of medical devices. The “armored-tank” strategy is realized by decoration of partially conformational transformed LZM (PCTL) assembly through oxidant-mediated process, followed by armoring with super-hydrophilic poly-2-methacryloyloxyethyl phosphorylcholine (pMPC). The outer armor of the “armored-tank” shows potent and durable zone defense against fibrinogen, platelet and bacteria adhesion, leading to long-term antithrombogenic properties over 14 days in vivo without anticoagulation. Additionally, the “fired” PCTL from “armored-tank” actively and effectively kills both Gram-positive and Gram-negative bacterial over 30 days as a supplement to the lacking bactericidal functions of passive outer armor. Overall, this “armored-tank” surface engineering strategy serves as a promising solution for preventing biofouling and thrombotic occlusion of medical devices.