Flexible pivoting of dynamin pleckstrin homology domain catalyzes fission: insights into molecular degrees of freedom
Krishnakanth Baratam, Kirtika Jha, Anand Srivastava
Abstract
-containing membranes modulates the lipids toward fission-favoring conformations and softens the membrane, and 2) PHD associates with membrane in multiple orientations using variable loops as pivots. We identify a new loop (VL4), which acts as an auxiliary pivot and modulates the orientation flexibility of PHD on the membrane-a mechanism that we believe may be important for high-fidelity dynamin collar assembly. Together, these insights provide a molecular-level understanding of the catalytic role of PHD in dynamin-mediated membrane fission.
Topics & Concepts
Pleckstrin homology domainBiologyDynaminMembraneGTPaseCell biologySynaptic vesicle recyclingSynaptic vesicleBiophysicsVesicleBiochemistryEndocytosisCellLipid Membrane Structure and BehaviorCellular transport and secretionDiffusion and Search Dynamics