Conservation of molecular responses upon viral infection in the non-vascular plant Marchantia polymorpha
Eric Ros-Moner, Tamara Jiménez‐Góngora, Luis Villar-Martín, Lana Vogrinec, Víctor M González, Denis Kutnjak, Ignacio Rubio‐Somoza
Abstract
After plants transitioned from water to land around 450 million years ago, they faced novel pathogenic microbes. Their colonization of diverse habitats was driven by anatomical innovations like roots, stomata, and vascular tissue, which became central to plant-microbe interactions. However, the impact of these innovations on plant immunity and pathogen infection strategies remains poorly understood. Here, we explore plant-virus interactions in the bryophyte Marchantia polymorpha to gain insights into the evolution of these relationships. Virome analysis reveals that Marchantia is predominantly associated with RNA viruses. Comparative studies with tobacco mosaic virus (TMV) show that Marchantia shares core defense responses with vascular plants but also exhibits unique features, such as a sustained wound response preventing viral spread. Additionally, general defense responses in Marchantia are equivalent to those restricted to vascular tissues in Nicotiana, suggesting that evolutionary acquisition of developmental innovations results in re-routing of defense responses in vascular plants. Assessment of the evolution of plant-virus interactions suggests rapid reshaping of plant viromes after land colonization, along with rerouting of general defense responses to newly acquired vascular tissues that became focal for viral infections.