Metal-Ion-Responsive Chromogenic Probe for Rapid, On-Location Detection of Foodborne Bacterial Pathogens in Contaminated Food Items
Nilanjan Dey
Abstract
An amphiphilic chromogenic probe based on an oxidized di(indolyl)arylmethane backbone has been utilized for visual detection of both Cu2+ (detection limit = 8.5 ppb) and Hg2+ (detection limit = 10.2 ppb) ions via mutually independent sensing pathways. The Cu2+ ion binds to the carboxylate ends (donor site) and induces a color change from orange to yellow in the aqueous medium, while coordinating Hg2+ at the bisindolyl moiety (acceptor site) can result in the formation of a red-colored solution. Interestingly, by selecting the proper excitation channel, we can specifically excite either the monomer species or nanoaggregates. The addition of Hg2+ enhances the monomer fluorescence, while Cu2+ induces quenching. However, in both cases, metal-ion coordination triggers dissociation of a preformed self-assembled structure. Further, the in-situ-formed Cu(II) complex was utilized for rapid, on-location detection of food-borne pathogens, such as Escherichia coli (E. coli) in contaminated food items and water (detection limit = 52 CFU·mL–1). E. coli induces reduction of Cu2+ to Cu+ and transforms the yellow-colored solution into an orange-colored solution. Finally, low-cost, reusable paper strips were designed as an eco-friendly, sustainable strategy to detect bacterial pathogens.