Three-Dimensional <i>In Vitro</i> Staphylococcus aureus Abscess Communities Display Antibiotic Tolerance and Protection from Neutrophil Clearance
Marloes I. Hofstee, Martijn Riool, Igors Terjajevs, Keith Thompson, Martin J. Stoddart, R. Geoff Richards, Sebastian A. J. Zaat, T. Fintan Moriarty
Abstract
Staphylococcus aureus is a prominent human pathogen in bone and soft-tissue infections. Pathophysiology involves abscess formation, which consists of central staphylococcal abscess communities (SACs), surrounded by a fibrin pseudocapsule and infiltrating immune cells. Protection against the ingress of immune cells such as neutrophils, or tolerance to antibiotics, remains largely unknown for SACs and is limited by the lack of availability of in vitro models. We describe a three-dimensional in vitro model of SACs grown in a human plasma-supplemented collagen gel.
Topics & Concepts
Staphylococcus aureusBiologyMicrobiologyIn vitroImmune systemAntibioticsPathogenAbscessImmunologyFibrinHuman pathogenBacteriaGeneticsBiochemistryStreptococcal Infections and TreatmentsAntimicrobial Resistance in StaphylococcusInfective Endocarditis Diagnosis and Management