Simultaneous estimation of voriconazole, moxifloxacin, and pirfenidone in rabbit lacrimal matrix using LC-MS/MS: an application to preclinical ocular pharmacokinetics
Amol Chhatrapati Bisen, Sristi Agrawal, Sachin Nashik Sanap, Anjali Mishra, Arpon Biswas, Sarvesh Kumar Verma, Rabi Sankar Bhatta
Abstract
multiple reaction monitoring mode. The sample extraction was performed using the protein precipitation method with minimal sample size. The validated methodology was employed to determine the ocular pharmacokinetics profile of marketed formulations containing VCZ, MOXI, and PIR in rabbit lacrimal matrix.
Topics & Concepts
ChromatographyMoxifloxacinProtein precipitationChemistryPharmacokineticsVoriconazoleMatrix (chemical analysis)BioanalysisAnalytePharmacologyAntibioticsAntifungalMedicineMicrobiologyBiologyBiochemistryOcular Infections and TreatmentsAntibiotics Pharmacokinetics and EfficacyBacterial Identification and Susceptibility Testing