Measuring DNA Damage Using the Alkaline Comet Assay in Cultured Cells
Elena Clementi, Zuzana Garajovà, Enni Markkanen
Abstract
) and methyl-methanesulfonate (MMS) or present endogenously in cells, and how to assess the repair kinetics after such an insult. The procedure described herein is easy to follow and allows the cost-effective assessment of single-strand breaks and their repair kinetics in cultured cells.
Topics & Concepts
DNA damageComet assayNucleoidDNADNA repairDNA fragmentationCell biologyMolecular biologyBiologyChemistryBiochemistryApoptosisProgrammed cell deathGeneEscherichia coliDNA Repair MechanismsCarcinogens and Genotoxicity AssessmentCRISPR and Genetic Engineering