SDC1-dependent TGM2 determines radiosensitivity in glioblastoma by coordinating EPG5-mediated fusion of autophagosomes with lysosomes
Zheng Wang, Qianping Chen, Hongxia Liu, Liang Zeng, Yuchuan Zhou, Xinglong Liu, Yang Bai, Jianghong Zhang, Yan Pan, Chunlin Shao
Abstract
; CQ: chloroquine; Cys-D: cystamine dihydrochloride; EPG5: ectopic P-granules 5 autophagy tethering factor; GBM: glioblastoma multiforme; GFP: green fluorescent protein; LAMP2: lysosomal associated membrane protein 2; LIRs: LC3-interacting regions; MAP1LC3/LC3: microtubule associated protein 1 light chain 3; NC: negative control; RFP: red fluorescent protein; RT: radiotherapy; SDC1: syndecan 1; SNAP29: synaptosome associated protein 29; SQSTM1/p62: sequestosome 1; STX17: syntaxin 17; TGM2: transglutaminase 2; TMT: tandem mass tag; VAMP8: vesicle associated membrane protein 8; WT: wild type.
Topics & Concepts
BiologyGlioblastomaRadiosensitivityAutophagyCell biologyCancer researchApoptosisGeneticsRadiation therapyInternal medicineMedicineAutophagy in Disease and TherapySignaling Pathways in DiseaseAdenosine and Purinergic Signaling