Optical clearing reveals TNBS-induced morphological changes of VGLUT2-positive nerve fibers in mouse colorectum
Tiantian Guo, Shivam Patel, Dhruv M. Shah, Ling Chi, Sharareh Emadi, David M. Pierce, Martin Han, Pablo R. Brumovsky, Bin Feng
2021American Journal of Physiology-Gastrointestinal and Liver Physiology18 citationsDOIOpen Access PDF
Abstract
We conducted genetic labeling and optical clearing to visualize extrinsic sensory nerve fibers in whole-mount colorectum, which revealed widespread presence of axons in the submucosal layer. Remarkably, axons in the submucosa were meandering and curvy, in contrast to axons in other layers generally aligned with the basal tissues. Intracolonic TNBS treatment led to pronounced changes of nerve fiber density and curviness, suggesting nerve fiber morphologies as potentially contributing factors to sensory sensitization.
Topics & Concepts
Myenteric plexusIrritable bowel syndromeSubmucosaNerve fiberMechanosensitive channelsAnatomyPathologyChemistryMedicineInternal medicineImmunohistochemistryIon channelReceptorGastrointestinal motility and disordersHelicobacter pylori-related gastroenterology studiesIon Channels and Receptors