Algorithmic Advances in Single Particle Cryo-EM Data Processing Using CryoSPARC
Ali Punjani
Abstract
Single particle cryo-EM (cryo-electron microscopy) allows high-resolution imaging of macromolecular complexes in close-to-native state, at near-atomic resolutions. Cryo-EM has undergone several technological breakthroughs in microscopy, electron detectors, and image processing that have enabled its use recently in solving high-resolution structures of difficult proteins and complexes. As cryo-EM makes rapid progress, several key challenges remain in the quest for higher resolutions on challenging targets, as well as in the widespread and routine use of cryo-EM. Here, we introduce new methods in the cryo-EM data processing pipeline, along with implementations within the cryoSPARC software system [1], to address some of these challenges.