Nanozyme Cascade Self-Powered H<sub>2</sub>O<sub>2</sub> Strategy for Chemiluminescence Array Sensor to Monitor and Deactivate Multiple Bacteria
Feng Shi, Haibing Zhu, Guiling Li, Maoying Peng, Ying Cao, Yanping Xia, Chuanli Ren, Juan Li, Zhanjun Yang
Abstract
Early warning and deactivation of multiple bacteria are highly desirable to prevent pathogen-responsible bacterial infectious illnesses. Here, we developed a nanozyme cascade self-powered H 2 O 2 strategy for a chemiluminescence (CL) array immunosensor to enable high-throughput and simultaneous monitoring of multiple bacteria as well as their deactivation. Specifically, a novel ZIF-67@CoFePBA yolk–shell nanozyme was synthesized through a dissociation and re-coordination mechanism, exhibiting significantly enhanced peroxidase (POD)-like activity due to the confinement and synergistic effects. ZIF-67@CoFePBA nanozyme was utilized to immobilize glucose oxidase (GOx) for constructing the nanozyme cascade self-powered H 2 O 2 system. ZIF-67@CoFePBA nanozyme can catalyze in-situ H 2 O 2 to produce hydroxyl radicals (·OH), resulting in stable glow-type CL to construct array immunosensors without exogenous H 2 O 2 . The self-powered CL array sensor was exploited to simultaneously detect numerous bacteria with wide linear ranges of 1.5×10–1.5×10 7 CFU/mL for Staphylococcus aureus and 1.5×10 2 –1.5×10 7 CFU/mL for Escherichia coli . Furthermore, the generated ·OH can destroy the internal structure of the bacteria and effectively eliminate them. This study provides a promising insight into the design of self-powered H 2 O 2 sensors for high-throughput and simultaneous detection of multiple bacteria and their subsequent deactivation.