Applications of Surface Plasmon Resonance and Biolayer Interferometry for Virus–Ligand Binding
Shruthi Murali, Richard R. Rustandi, Xiwei Zheng, Anne F. Payne, Liang Shang
Abstract
Surface plasmon resonance and biolayer interferometry are two common real-time and label-free assays that quantify binding events by providing kinetic parameters. There is increased interest in using these techniques to characterize whole virus-ligand interactions, as the methods allow for more accurate characterization than that of a viral subunit-ligand interaction. This review aims to summarize and evaluate the uses of these technologies specifically in virus-ligand and virus-like particle-ligand binding cases to guide the field towards studies that apply these robust methods for whole virus-based studies.
Topics & Concepts
Surface plasmon resonanceInterferometryLigand (biochemistry)Resonance (particle physics)ChemistryMaterials scienceNuclear magnetic resonanceVirologyOpticsNanotechnologyPhysicsNanoparticleBiologyReceptorBiochemistryAtomic physicsMonoclonal and Polyclonal Antibodies ResearchAdvanced Biosensing Techniques and ApplicationsAdvanced biosensing and bioanalysis techniques