CRISPR/dCas9-targeted H3K27me3 demethylation at the CUC3 boundary gene triggers ectopic transcription and impacts plant development

Abstract

locus induces ectopic transcription and gene expression patterns, leading to altered leaf morphology and meristem integrity. Combining molecular and phenotypic analyses, we thus establish evidence directly linking H3K27me3-mediated repression to developmental outcomes. Our study highlights locus-specific epigenetic editing as a powerful approach to dissect the functional impacts of histone modifications on transcription and morphogenesis, and provides a framework for unveiling the causal role of chromatin dynamics in plant developmental plasticity.

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