The Novel P <sub>II</sub> -Interacting Protein PirA Controls Flux into the Cyanobacterial Ornithine-Ammonia Cycle
Paul Bolay, Rokhsareh Rozbeh, M. Isabel Muro‐Pastor, Stefan Timm, Martin Hagemann, Francisco J. Florencio, Karl Forchhammer, Stephan Klähn
Abstract
Cyanobacteria contribute a significant portion to the annual oxygen yield and play important roles in biogeochemical cycles, e.g., as major primary producers. Due to their photosynthetic lifestyle, cyanobacteria also arouse interest as hosts for the sustainable production of fuel components and high-value chemicals. However, their broad application as microbial cell factories is hampered by limited knowledge about the regulation of metabolic fluxes in these organisms. Our research identified a novel regulatory protein that controls nitrogen flux, in particular arginine synthesis. Besides its role as a proteinogenic amino acid, arginine is a precursor for the cyanobacterial storage compound cyanophycin, which is of potential interest to biotechnology. Therefore, the obtained results will not only enhance our understanding of flux control in these organisms but also help to provide a scientific basis for targeted metabolic engineering and, hence, the design of photosynthesis-driven biotechnological applications.