The potential of combined mutation sequencing of plasma circulating cell‐free DNA and matched white blood cells for treatment response prediction
Paul van der Leest, Ed Schuuring
Abstract
Highly sensitive mutation detection methods enable the application of circulating cell-free DNA for molecular tumor profiling. Recent studies revealed that sequencing artifacts, germline variants, and clonal hematopoiesis confound the interpretation of sequencing results and complicate subsequent treatment decision making and disease monitoring. Parallel sequencing of matched white blood cells promises to overcome these issues and enables appropriate variant calling. Comment on: https://doi.org/10.1002/1878-0261.12617.
Topics & Concepts
BiologyGermline mutationDNA sequencingGermlineCell-free fetal DNAMutationComputational biologySingle cell sequencingHaematopoiesisGeneticsDNAExome sequencingStem cellGenePrenatal diagnosisFetusPregnancyCancer Genomics and DiagnosticsSingle-cell and spatial transcriptomicsLung Cancer Treatments and Mutations