Monitoring bacterial spore metabolic activity using heavy water-induced Raman peak evolution
Rasmus Öberg, Tobias Dahlberg, Dmitry Malyshev, Magnus Andersson
Abstract
To detect and monitor bacterial spore germination and outgrowth into bacterial cells, we incubate bacterial spores with heavy water. By tracking the induced C–D Raman peak as the spore starts to metabolize water, we get an indication of whether germination has begun.
Topics & Concepts
SporeEndosporeGerminationBacillus cereusSpore germinationBacteriaCereusFood scienceRaman spectroscopySterilization (economics)ChemistryMetabolic activityFood spoilageIncubationMicrobiologyBiologyBiochemistryBotanyPhysiologyForeign exchangePhysicsMonetary economicsEconomicsForeign exchange marketGeneticsOpticsSpectroscopy Techniques in Biomedical and Chemical ResearchMetabolomics and Mass Spectrometry StudiesBacillus and Francisella bacterial research