Overcoming Culture Restriction for SARS-CoV-2 in Human Cells Facilitates the Screening of Compounds Inhibiting Viral Replication
Santseharay Ramírez, Carlota Fernandez-Antunez, Andrea Galli, Alexander P. Underwood, Long V. Pham, Line A. Ryberg, Shan Feng, Martin Schou Pedersen, Lotte S. Mikkelsen, Sandrine Belouzard, Jean Dubuisson, Christina Sølund, Nina Weis, Judith M. Gottwein, Ulrik Fahnøe, Jens Bukh
Abstract
Efforts to mitigate the coronavirus disease 2019 (COVID-19) pandemic include the screening of existing antiviral molecules that could be repurposed to treat severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections. Although SARS-CoV-2 replicates and propagates efficiently in African green monkey kidney (Vero) cells, antivirals such as nucleos(t)ide analogs (nuc’s) often show decreased activity in these cells due to inefficient metabolization.
Topics & Concepts
VirologyViral replicationSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Replication (statistics)2019-20 coronavirus outbreakCoronavirus disease 2019 (COVID-19)Sars virusBiologyCell cultureComputational biologyVirusMedicineGeneticsInfectious disease (medical specialty)PathologyOutbreakDiseaseSARS-CoV-2 and COVID-19 ResearchViral gastroenteritis research and epidemiologySARS-CoV-2 detection and testing