Electrochemical Detection of H<sub>2</sub>O<sub>2</sub> on Graphene Nanoribbons/Cobalt Oxide Nanorods‐Modified Electrode
Preethika Murugan, Ashok K. Sundramoorthy, Ramila D. Nagarajan, Raji Atchudan, S. Rajeshkumar, Dhanraj Ganapathy, Sandeep Arya, Asma A. Alothman, Mohamed Ouladsmane
Abstract
The most important biological changes which have to be monitored is the mechanism of ageing in the human body where the mitochondria play a major role. Hydrogen peroxide (H 2 O 2 ) is one of the important markers for the reactive oxygen species (ROS), which denatures the protein and DNA, that was the main contributory factor of ageing. So, it is very important to monitor H 2 O 2 levels in the biological samples. Herein, we reported the preparation of 1D graphene nanoribbon/cobalt oxide nanorod (GNR/Co 3 O 4 ) based nanocomposite‐modified electrochemical sensor for H 2 O 2 . Firstly, GNR was synthesized by oxidative unzipping of multiwalled carbon nanotubes (MWCNTs). Secondly, cobalt oxide nanorods (Co 3 O 4 ) were grown onto GNR by a chemical reduction process. As‐prepared nanocomposite was characterized by UV‐Visible spectroscopy (UV‐Vis) and HR‐TEM. Electrochemical properties of GNR/Co 3 O 4 ‐coated electrode were studied by cyclic voltammetry (CV) which showed two redox peaks at 0.93 and 0.88 V in phosphate buffer solution. Next, the electrocatalytic activity of GNR/Co 3 O 4 ‐coated electrode was studied against H 2 O 2 oxidation. The electrochemical studies revealed that GNR/Co 3 O 4 ‐coated electrode exhibited high electrocatalytic activity for H 2 O 2 oxidation at 0.925 V. This sensor showed a linear response for H 2 O 2 oxidation from 10 to 200 μ M. The limit of detection (LOD) was calculated to be 1.27 μ M. The selectivity of the sensor was also studied with other biomolecules associated in the human body, and the results showed that interference effect is negligible. Thus, the proposed GNR/Co 3 O 4 ‐modified electrode can be used for H 2 O 2 detection with excellent stability and selectivity.