Target-mediated self-assembly of DNA networks for sensitive detection and intracellular imaging of APE1 in living cells
Xue‐Mei Zhou, Ying Zhuo, Ruo Yuan, Ya-Qin Chai
Abstract
. More importantly, the aptamer Sgc8 assembled on f-TDNs could enhance the targeting activity of the DNA structure to tumor cells, allowing it to endocytose into cells without any transfection reagents, which could achieve selective imaging of intracellular APE1 in living cells. Meanwhile, the siRNA carried by f-TDN1 could be accurately released to promote tumor cell apoptosis in the presence of endogenous target APE1, realizing effective and precise tumor therapy. Benefiting from the high specificity and sensitivity, the developed DNA nanostructures provide an excellent nanoplatform for precise cancer diagnosis and therapy.
Topics & Concepts
TransfectionIntracellularDNABiophysicsAptamerDetection limitCancer cellFluorescenceApoptosisFluorescence-lifetime imaging microscopyChemistryEndonucleaseMolecular biologyCell biologyNanotechnologyCancerMaterials scienceBiologyGeneBiochemistryPhysicsGeneticsQuantum mechanicsChromatographyAdvanced biosensing and bioanalysis techniquesRNA Interference and Gene DeliveryDNA and Nucleic Acid Chemistry