2′-O methylation of RNA cap in SARS-CoV-2 captured by serial crystallography
Mateusz Wilamowski, D.A. Sherrell, G. Minasov, Youngchang Kim, L. Shuvalova, Alex Lavens, Ryan Chard, N. Maltseva, R. Jedrzejczak, Mónica Rosas‐Lemus, Nickolaus Saint, Ian Foster, K. Michalska, K.J.F. Satchell, A. Joachimiak
Abstract
Significance The 2′-O methyl group in Cap-1 is essential to protect viral RNA from host interferon-induced response. We determined crystal structures of SARS-CoV-2 Nsp10/16 heterodimer in complex with substrates (Cap-0 analog and S-adenosyl methionine) and products (Cap-1 analog and S-adenosyl-L-homocysteine) at room temperature using synchrotron serial crystallography. Analysis of these structures will aid structure-based drug design against 2′-O-methyltransferase from SARS-CoV-2.
Topics & Concepts
MethylationMethyltransferaseRNAChemistryMethionineSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)Messenger RNACrystal structureCrystallographyStereochemistryMolecular biologyCoronavirus disease 2019 (COVID-19)VirologyBiologyBiochemistryMedicineDNAInfectious disease (medical specialty)GeneDiseaseAmino acidPathologyRNA modifications and cancerRNA and protein synthesis mechanismsBiochemical and Molecular Research