Sphingobium estronivorans sp. nov. and Sphingobium bisphenolivorans sp. nov., isolated from a wastewater treatment plant

Abstract

Two Gram-stain-negative, aerobic, motile and rod-shaped bacteria, one designated as strain AXB T , capable of degrading estrogens, and another, YL23 T , capable of degrading estrogen and bisphenol A, were isolated from activated sludge in Xiamen City, PR China. The optimum temperature and pH of both strains were 25–35 °C and pH 7.0–8.0. While strain AXB T could tolerate 3 % (w/v) NaCl, YL23 T could only grow between 0–1 % (w/v) NaCl. They contained ubiquinone-10 as the major quinone, spermidine as the major polyamine, summed feature 8 (comprising C 18:1 ω6 c and/or C 18:1 ω7 c ) as the major fatty acids and diphosphatidylglycerol, phosphatidylcholine, phosphatidyldimethylethanolamine, phosphatidylethanolamine, phosphatidylglycerol and sphingoglycolipid as the major polar lipids. The DNA G+C contents of strains AXB T and YL23 T were 63.6 and 63.7 mol%, respectively. Based on the results of 16S rRNA gene sequence analysis, strains AXB T and YL23 T belonged to the genus Sphingobium . Strain AXB T was most closely related to Sphingobium chlorophenolicum NBRC 16172 T (97.5 %) and Sphingobium chungbukense DJ77 T (97.2 %), and strain YL23 T was most closely related to S. chlorophenolicum NBRC 16172 T (97.4 %) and S. quisquiliarum P25 T (97.1 %). Average nucleotide identity values between these two strains and S. chlorophenolicum NBRC 16172 T , S. chungbukense DJ77 T , Sphingobium chinhatense IP26 T , Sphingobium quisquiliarum P25 T and Sphingobium japonicum UT26S T were from 80.7 to 85.8 %. In conclusion, strains AXB T and YL23 T represent novel species of the genus Sphingobium , for which the names Sphingobium estronivorans sp. nov. and Sphingobium bisphenolivorans sp. nov. are proposed, respectively. The type strains of S. estronivorans and S. bisphenolivorans are AXB T (=MCCC 1K01232 T =DSM 102173 T ) and YL23 T (=MCCC 1K02300 T =DSM 102172 T ), respectively.

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