Mapping paratopes of nanobodies using native mass spectrometry and ultraviolet photodissociation
Luis A. Macias, Xun Wang, Bryan W. Davies, Jennifer S. Brodbelt
Abstract
nanobody·antigen complexes empowered assignment of nanobody paratopes and intermolecular salt-bridges, elevating the capabilities of UVPD as a new strategy for characterization of nanobodies.
Topics & Concepts
PhotodissociationUltravioletMass spectrometryChemistryChromatographyPhotochemistryMaterials scienceOptoelectronicsMass Spectrometry Techniques and ApplicationsIon-surface interactions and analysisAdvanced Proteomics Techniques and Applications