Dysregulation of the WNK4-SPAK/OSR1 pathway has a minor effect on baseline NKCC2 phosphorylation
Yujiro Maeoka, Luan T. Nguyen, Avika Sharma, Ryan J. Cornelius, Xiao‐Tong Su, Marissa Gutierrez, Héctor Carbajal‐Contreras, María Castañeda‐Bueno, Gerardo Gamba, James A. McCormick
Abstract
The renal cation cotransporters NCC and NKCC2 are activated following phosphorylation mediated by the WNK4-SPAK/OSR1 pathway. While disruption of this pathway strongly affects NCC activity, effects on NKCC2 activity are unclear since the commonly used phospho-NKCC2 antibody was recently reported to cross-react with phospho-NCC in mice on the C57BL/6 background. Using a new phospho-NKCC2 antibody specific for C57BL/6, we show that inhibition or activation of the WNK4-SPAK/OSR1 pathway in mice only mildly affects NKCC2 phosphorylation.
Topics & Concepts
PhosphorylationKinaseChemistryCell biologyBiochemistryBiologyIon Transport and Channel RegulationDiet, Metabolism, and DiseaseIon channel regulation and function