Multiplexed transcriptional repression identifies a network of bactericidal interactions between mycobacterial respiratory complexes
Matthew B. McNeil, Heath W. K. Ryburn, Justin F. Tirados, Chen‐Yi Cheung, Gregory M. Cook
Abstract
as a physiological and molecular model for mycobacterial respiration to identify interactions between respiratory complexes. In this study, we identified synthetic lethal and synergistic interactions between respiratory complexes and demonstrated how the engineering of CRISPRi-guide sequences can be used to further explore networks of interacting gene pairs. These results provide fundamental insights into the functions of and interactions between bioenergetic complexes and the utility of CRISPRi in designing drug combinations.
Topics & Concepts
Mycobacterium smegmatisPsychological repressionComputational biologyBiologyBioenergeticsDrug discoveryMycobacterium tuberculosisTuberculosisMicrobiologyChemistryGeneBioinformaticsGeneticsMedicineGene expressionMitochondrionPathologyTuberculosis Research and EpidemiologyMycobacterium research and diagnosisAntibiotic Resistance in Bacteria