A Point Mutation in the Transcriptional Repressor PerR Results in a Constitutive Oxidative Stress Response in Clostridioides difficile 630Δ <i>erm</i>
Daniel Troitzsch, Hao Zhang, Silvia Dittmann, Dorothee Düsterhöft, Timon Alexander Möller, Annika‐Marisa Michel, Lothar Jänsch, Katharina Riedel, José Manuel Borrero‐de Acuña, Dieter Jahn, Susanne Sievers
Abstract
The intestinal pathogen Clostridioides difficile is one of the major challenges in medical facilities nowadays. In order to better combat the bacterium, detailed knowledge of its physiology is mandatory. C. difficile strain 630Δ erm was generated in a laboratory from the patient-isolated strain C. difficile 630 (H. A. Hussain, A. P. Roberts, and P. Mullany, J Med Microbiol 54:137–141, 2005, https://doi.org/10.1099/jmm.0.45790-0 ) and represents a reference strain for many researchers in the field, serving as the basis for the construction of insertional gene knockout mutants using the ClosTron system (J. T. Heap, O. J. Pennington, S. T. Cartman, G. P. Carter, and N. P. Minton, J Microbiol Methods 70:452–464, 2007, https://doi.org/10.1016/j.mimet.2007.05.021 ).