Rapid and sensitive detection of <i>Staphylococcus aureus via</i> an all-in-one staggered strand exchange amplification platform
Jian Zhang, Xiangning Han, Ye Wang, Xiaofeng Mu, Chao Shi, Yong Li, Cuiping Ma
Abstract
A staggered strand exchange amplification (SSEA) method was developed by employing a DNA polymerase and two sets of forward and reverse primers arranged in tandem that invade the denaturation bubbles of double-stranded DNA.
Topics & Concepts
Staphylococcus aureusDenaturation (fissile materials)DNA extractionPolymerase chain reactionPathogenDNAFood poisoningMicrobiologyFood safetyChemistryBiologyChromatographyFood scienceBacteriaGeneBiochemistryGeneticsNuclear chemistryBiosensors and Analytical DetectionAdvanced biosensing and bioanalysis techniquesIdentification and Quantification in Food