Schisandrin A from Schisandra chinensis Attenuates Ferroptosis and NLRP3 Inflammasome‐Mediated Pyroptosis in Diabetic Nephropathy through Mitochondrial Damage by AdipoR1 Ubiquitination
Xiaohu Wang, Qin Li, Bangzhi Sui, Maodi Xu, Zhichen Pu, Teng Qiu
Abstract
Schisandra chinensis , as a Chinese functional food, is rich in unsaturated fatty acids, minerals, vitamins, and proteins. Hence, this study was intended to elucidate the effects and biological mechanism of Schisandrin A from Schisandra chinensis in DN. C57BL/6 mice were fed with a high‐fat diet and then injected with streptozotocin (STZ). Human renal glomerular endothelial cells were stimulated with 20 mmol/L d‐glucose for DN model. Schisandrin A presented acute kidney injury in mice of DN. Schisandrin A reduced oxidative stress and inflammation in model of DN. Schisandrin A reduced high glucose‐induced ferroptosis and reactive oxygen species (ROS‐)‐mediated pyroptosis by mitochondrial damage in model of DN. Schisandrin A directly targeted AdipoR1 protein and reduced LPS+ATP‐induced AdipoR1 ubiquitination in vitro model. Schisandrin A activated AdipoR1/AMPK signaling pathway and suppressed TXNIP/NLRP3 signaling pathway in vivo and in vitro model of DN. Conclusively, our study revealed that Schisandrin A from Schisandra chinensis attenuates ferroptosis and NLRP3 inflammasome‐mediated pyroptosis in DN by AdipoR1/AMPK‐ROS/mitochondrial damage. Schisandrin A is a possible therapeutic option for DN or other diabetes.