Two new 19-hydroxy bufadienolides with cytotoxic activity from the skins of <i>Bufo melanosticus</i>
Lingjie Meng, Sanhua Li, Qinghong Kong, Mei Wang, Xiaoke Zhang, Xinting Zhu, Wei Yu, Nian Jiang, Zeli Chun, Li Nian, Yun Liu
Abstract
Two new 19-hydroxy bufadienolides, named bufohydrolide A (1) and bufohydrolide B (2), and four known analogs (3-6) were isolated from the aqueous extracts of the skins of Bufo melanosticus. Their structures were established by spectral data analyses, such as UV, IR, 1 D/2D NMR and mass spectra. Compounds 1-6 were evaluated for their cytotoxic activity against SMMC-7721, HT-29 and A549 cells. Noteworthily, all six isolates exhibited various levels of anti-proliferative activities with IC50 values ranging from 0.02 ± 0.0002 to 25 ± 0.5 μM.
Topics & Concepts
Cytotoxic T cellStereochemistryChemistryIC50In vitroBiochemistryPhytochemistry and Bioactive CompoundsNatural product bioactivities and synthesisPhytochemical Studies and Bioactivities