Primary Amine-Induced Coacervation in Alkyl Polyglucoside Micellar Solution for Supramolecular Solvent-Based Microextraction
Yanina Kovalchuk, Christina Vakh, Evgenia A. Safonova, Andrey Bulatov
Abstract
A phenomenon of supramolecular solvent formation in a micellar solution of alkyl polyglucoside in the presence of primary amine is presented for the first time. Primary amines were investigated as coacervation agents for supramolecular solvent formation based on alkyl polyglucosides (C 8 –C 10 and C 8 –C 14 ). It was found that addition of primary amine promoted in situ formation of tiny supramolecular solvent droplets in alkaline media followed by the separation of a water-immiscible liquid phase. The phenomenon of the formation of supramolecular solvents from an alkyl polyglucoside in an alkaline medium is presented for the first time. The phase diagrams for ternary systems based on alkyl polyglucoside-primary amine-water and physicochemical properties of the supramolecular solvents were investigated. The proposed supramolecular solvents were studied for the microextraction of sulfonamide antibiotics from biological fluids. The salting-out effect on mass-transfer was also investigated. Alkyl polyglucoside C 8 –C 10 and 1-heptylamine provided more effective preconcentration of the antibiotics in the presence of a salting-out agent (sodium carbonate) among studied amphiphiles and coacervation agents. The microextraction procedure was applied to the chromatographic determination of antibiotics in spiked human plasma and serum samples. The limits of detection, calculated from a blank test based on 3σ, were 20 μg L –1 for all analytes.