Principles for Rigorous Design and Application of Synthetic Microbial Communities
Yu‐Xiao Zhang, Minyu Jing, Lihui Lyu, Li Nie, Xihui Xu, Rao Sun, Xiyuan Xu, Siyu Chen, Shuobing He, Yumeng Zhang, Ping Huang, Weijie Luo, Jiaojiao Liang, Gui‐Feng Gao, Kunkun Fan, Teng Yang, Liyan Zhang, Xiao Fu, Sarah M. Allard, Jack A. Gilbert, Jiabao Zhang, Haiyan Chu
Abstract
Synthetic microbial communities (SynComs) are microbial consortia with defined taxonomic and functional traits, so that the combination elicits a predictable response under defined conditions. SynComs are artificially designed to enable inter-species metabolic interactions, metabolic division of labor, and ecological interactions that can elicit phenotypes like colonization stability and environmental adaptation. As an applied tool, SynComs have been deployed in diverse contexts, including agriculture, industry, and environmental ecology. This systematic review explores the processes used to construct SynComs, the mechanisms of metabolic interaction between members, and a review of the different ways that SynComs have been applied. We also explore the challenges for SynCom development and application, and future research directions that could overcome these challenges. SynComs are a powerful tool in our arsenal of applied technologies, but research and application are still nascent. While advances have been made, more research is needed to ensure SynCom technologies do not threaten global ecological security. SynCom technology represents a versatile platform for the controlled manipulation of microbial systems, enabling targeted modification of ecological and physiological processes. This emerging field marks a transition from descriptive biology toward a predictive and engineering-driven framework for understanding and shaping living systems.