Four-core fiber-based multi-tapered WaveFlex biosensor for rapid detection of Vibrio parahaemolyticus using nanoparticles-enhanced probes
Qianqian Fu, Yiyan Xie, Feilong Gao, Ragini Singh, Xinlong Zhou, Bingyuan Zhang, Santosh Kumar
Abstract
Infections caused by Vibrio parahaemolyticus ( V. parahaemolyticus ) can be highly fatal, making rapid and sensitive detection of them is essential. A new optical fiber biosensor based on localized surface plasmon resonance (LSPR) phenomenon is developed in this paper. A tapered-in-tapered fiber structure based on MFM is constructed by using four-core fiber (FCF) and multi-mode fiber (MMF) to qualitatively detect different concentrations of V. parahaemolyticus . The sensor successfully excites the LSPR phenomenon and increases the attachment point of biomolecules on the probe surface by fixing gold nanoparticles (AuNPs), molybdenum disulfide nanoparticles (MoS 2 -NPs) and cerium dioxide nanorods (CeO 2 -NRs). The functionalization of polyclonal antibodies on the probe surface can improve the specificity of the sensor. The linear detection range of the developed sensor was 1 × 10 0 -1 × 10 7 CFU/mL, the sensitivity was 1.61 nm/[CFU/mL], and the detection limit was 0.14 CFU/mL. In addition, the reusability, reproducibility, stability, and selectivity of the sensor probe are also tested, which shows that the sensor has great application prospects.