Characterization and human microfold cell assay of fish oil microcapsules: Effect of spray drying and freeze-drying using konjac glucomannan (KGM)-soybean protein isolate (SPI) as wall materials
Tingting Cui, Caijiao Chen, Airong Jia, Dong‐Dong Li, Yaping Shi, Miansong Zhang, Xinfeng Bai, Xue Liu, Changheng Liu
Abstract
The properties of microencapsulated fish oil, prepared with spray drying and freeze-drying processes using emulsions of konjac glucomannan (KGM) and soybean protein isolate (SPI) was investigated. Comparative analysis of microcapsules showed the encapsulation efficiency of spray drying and freeze-drying were 90.10% and 83.52% respectively. Scanning electron microscopy (SEM) and confocal laser scanning microscopy (CLSM) results exhibited that microcapsules prepared with spray drying were spherical particles with a compact structure, while microcapsules prepared with freeze-drying were irregular particles with a pore-like structure. Release kinetics test further indicated retention rate of core materials for microcapsules prepared with spray drying were better than with freeze-drying. In addition, a human epithelial microfold cell (M-cell) transcytotic assay demonstrated that the M-cells had greater transport activity for the exogenous microcapsules.