Fluorescence Resonance Energy Transfer-Based Aptasensor Made of Carbon-Based Nanomaterials for Detecting Lactoferrin at Low Concentrations
Yingqi Zhang, Jin Zhang
Abstract
) is 365 nm. Due to the interaction of the aptamer and GO through the π-π* interaction, GO can approach CDs, resulting in FRET quenching. In the presence of lactoferrin, the fluorescence intensity of CDs-aptamer is restored as the binding affinity between lactoferrin and the aptamer is stronger than the π-π* interaction between the aptamer and GO. A linear relationship between the restored fluorescence intensity and the concentration of lactoferrin in artificial saliva with a range from 4 to 16 μg/mL is observed. The limit of detection of the solution-based aptasensor is estimated at 2.48 μg/mL. In addition, the sensing performance of the aptasensor made of carbon nanomaterials has been evaluated to test different proteins including major salivary proteins. The results show that this aptasensor has a high selectivity to detect LF with a low concentration, <16 μg/mL.