Inhibition of DNA methylation in proliferating human lymphoma cells by immune cell oxidants
Karina M. O'Connor, Andrew B. Das, Christine C. Winterbourn, Mark B. Hampton
Abstract
, significantly inhibited DNA methylation. Both oxidants inhibited DNA methyltransferase 1 activity, but only chloramine depleted SAM, suggesting that removal of substrate was the most effective means of inhibiting DNA methylation. These results indicate that immune cell-derived oxidants generated during inflammation have the potential to affect the epigenome of neighboring cells.
Topics & Concepts
DNA methylationMethylationImmune systemMethyltransferaseJurkat cellsChemistryMolecular biologyEpigeneticsDNA damageDNABiologyDNA methyltransferaseBiochemistryT cellGene expressionImmunologyGeneEpigenetics and DNA MethylationImmune Cell Function and InteractionImmune cells in cancer