Next-generation Sequencing Reveals Age-dependent Genetic Underpinnings in Lung adenocarcinoma
Xiaonan Wu, Jun Zhao, Ling Yang, Xin Nie, Zheng Wang, Ping Zhang, Chao Li, Xueqing Hu, Min Tang, Yuting Yi, Xinhua Du, Xuefeng Xia, Yanfang Guan, Zicheng Yu, Wenguang Gu, Xiangming Quan, Lin Li, Hong Shi
Abstract
Background: More than 40% of lung cancer patients are diagnosed at ages over 70. However, the genomic and clinical characteristics among them remain elusive. Here, we performed targeted capture sequence to characterize the mutational spectrum of Chinese lung adenocarcinoma (LUAD) patients across ages. Patients and Methods: 2025 LUAD patients were divided into three groups: young (50 years old) (n=416, 20.54%), intermediate (51~69 years old) (n=1271, 62.77%), and aged (70 years old) (n=338, 16.69%). 1,021-gene panel and 59-gene panel were used for sequencing with tissue samples. Genetic alterations and tumor mutation burden (TMB) in LUAD patients were investigated.