DEqMS: A Method for Accurate Variance Estimation in Differential Protein Expression Analysis
Yafeng Zhu, Lukas M. Orre, Yan Zhou Tran, Georgios Mermelekas, Henrik J. Johansson, Alina Malyutina, Simon Anders, Janne Lehtiö
Abstract
proteome spike-in data, using both label-free and TMT-labeled quantification. Compared with previous statistical methods used in quantitative proteomics, DEqMS showed consistently better accuracy in detecting altered protein levels compared with other statistical methods in both label-free and labeled quantitative proteomics data. DEqMS is available as an R package in Bioconductor.
Topics & Concepts
Expression (computer science)Variance (accounting)Protein expressionEstimationDifferential (mechanical device)Computational biologyComputer scienceStatisticsMathematicsBiologyGeneticsEngineeringGeneBusinessAccountingAerospace engineeringSystems engineeringProgramming languageGene expression and cancer classificationAdvanced Proteomics Techniques and ApplicationsBioinformatics and Genomic Networks