Antibody-mediated disruption of the SARS-CoV-2 spike glycoprotein
Antoni G. Wrobel, D.J. Benton, Saira Hussain, Ruth Harvey, Stephen R. Martin, Chloë Roustan, Peter B. Rosenthal, J.J. Skehel, S.J. Gamblin
Abstract
The CR3022 antibody, selected from a group of SARS-CoV monoclonal antibodies for its ability to cross-react with SARS-CoV-2, has been examined for its ability to bind to the ectodomain of the SARS-CoV-2 spike glycoprotein. Using cryo-electron microscopy we show that antibody binding requires rearrangements in the S1 domain that result in dissociation of the spike.
Topics & Concepts
EctodomainGlycoproteinAntibodyMonoclonal antibodySpike (software development)VirologySevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2)CoronavirusChemistryCoronavirus disease 2019 (COVID-19)BiologyCell biologyMolecular biologyImmunologyMedicineReceptorBiochemistryComputer scienceDiseaseInfectious disease (medical specialty)Software engineeringPathologySARS-CoV-2 and COVID-19 ResearchViral gastroenteritis research and epidemiologyAnimal Virus Infections Studies