Priority changes between biofilm exopolysaccharides synthesis and rhamnolipids production are mediated by a c-di-GMP-specific phosphodiesterase NbdA in Pseudomonas aeruginosa
Shixun Liu, Anming Xu, Bin Xie, Fengxue Xin, Weiliang Dong, Jie Zhou, Min Jiang
Abstract
. Further genomic, transcriptomic analyses and gene deletion revealed that downregulation of c-di-GMP level was the key to switch biofilm exopolysaccharides accumulation to RLs surge, by transcriptionally upregulating a c-di-GMP phosphodiesterase NbdA. Overall, this study demonstrates the importance of c-di-GMP in coordinating biofilm exopolysaccharides and RLs synthesis, and provides an inspiration for enhancing RLs production through regulating c-di-GMP level.
Topics & Concepts
BiofilmPseudomonas aeruginosaMutantPhosphodiesteraseOverproductionBacteriaMicrobiologyChemistryDownregulation and upregulationPhenotypePolysaccharidePseudomonadalesPseudomonasPseudomonadaceaeSecretionGeneCell biologyBiochemistryBiologyGeneticsEnzymeBacterial biofilms and quorum sensingMicrobial Community Ecology and PhysiologyBacterial Genetics and Biotechnology