Portable and simultaneous detection of four respiratory pathogens through a microfluidic LAMP and real-time fluorescence assay
Junwen Liu, Zhi Zeng, Fei-Na Li, Bo Jiang, You Nie, Guohao Zhang, Biao Pang, Lin Sun, Rongzhang Hao
Abstract
copies per mL. The test results on clinical samples demonstrated that the developed method has high sensitivity (92.00%) and high specificity (100.00%) and even has the capability to differentiate mixed-infection samples. With simple operation and high detection efficiency, the present portable and simultaneous detection assay could significantly improve the efficiency of on-site detection of respiratory infectious diseases and promote the accurate treatment, efficient prevention and control of the diseases.
Topics & Concepts
MicrofluidicsRespiratory systemChromatographyFluorescenceMicrobiologyBiologyChemistryMedicineNanotechnologyMaterials scienceOpticsPhysicsInternal medicineBiosensors and Analytical DetectionAdvanced biosensing and bioanalysis techniquesRespiratory viral infections research