New triorganotin(<scp>iv</scp>) compounds with aromatic carboxylate ligands: synthesis and evaluation of the pro-apoptotic mechanism
Faisal Rashid, Noor Uddin, Saqib Ali, Ali Haider, S.A. Tirmizi, Paula L. Diaconescu, Jamshed Iqbal
Abstract
cisplatin. The cytotoxic effect of the most potent compound C2 was confirmed by LDH cytotoxicity assay and fluorescence imaging after PI staining. Apoptotic features in compound C2 treated cancer cells were visualized after DAPI staining while regulation of apoptosis was observed by reactive oxygen species generation, binding of C2 with DNA, a change in mitochondrial membrane potential and expression of activated caspase-9 and caspase-3 in cancer cells. Results are indicative of activation of the intrinsic pathway of apoptosis in C2 treated cancer cells.
Topics & Concepts
CarboxylateChemistryMechanism (biology)ApoptosisStereochemistryCombinatorial chemistryBiochemistryPhysicsQuantum mechanicsOrganometallic Compounds Synthesis and CharacterizationMetal complexes synthesis and propertiesCrystal structures of chemical compounds